uropathogenic escherichia coli strain  (ATCC)

Journal: iScience

Article Title: Obesity promotes urinary tract infection by disrupting bladder focal adhesion kinase signaling

doi: 10.1016/j.isci.2025.113862

Figure Lengend Snippet: Obesity increases the uropathogenic E. coli burden in female mice (A–C) Body weight (A), non-fasting blood glucose (B), and blood glucose concentrations following intraperitoneal glucose tolerance testing (C) are measured in female C57BL/6 mice maintained on standard chow (SC) or a high-fat diet (HFD) for 4, 8, or 12 weeks. Graphs show the mean values and SEM. (D) Urine and bladder UPEC (UTI89) burden is enumerated after transurethral UTI at the indicated time points. HPI notes hours post-infection. The horizontal line indicates the geometric mean of each group. The dotted line indicates the limits of detection. (A–D) Each data point represents a measurement from an individual animal ( n = 11–17 mice/cohort). Asterisks indicate significant p- values for the indicated pairwise comparison (Mann-Whitney U test). ∗ p < 0.05, ∗∗ p < 0.01, and ∗∗∗∗ p < 0.0001.

Article Snippet: Uropathogenic Escherichia coli CFT073 , ATCC , Cat#700928.

Techniques: Infection, Comparison, MANN-WHITNEY

Journal: iScience

Article Title: Obesity promotes urinary tract infection by disrupting bladder focal adhesion kinase signaling

doi: 10.1016/j.isci.2025.113862

Figure Lengend Snippet: Obesity increases the uropathogenic E. coli burden in male mice (A–C) Body weight (A), non-fasting blood glucose (B), and blood glucose concentrations following intraperitoneal glucose tolerance testing (C) are measured in male C57BL/6 mice maintained on a standard chow (SC) or a high-fat diet (HFD) for 8 or 12 weeks. Graphs show the mean values and SEM. (D) Urine and bladder UPEC (UTI89) burden is enumerated after direct bladder inoculation at the indicated time points. HPI notes hours post-infection. The horizontal line indicates the geometric mean of each group. The dotted line indicates the limits of detection. (A–D) Each data point represents a measurement from an individual animal ( n = 11–19 mice/cohort). Asterisks indicate significant p- values for the indicated pairwise comparison (Mann-Whitney U test). ∗ p < 0.05, ∗∗ p < 0.01, and ∗∗∗∗ p < 0.0001.

Article Snippet: Uropathogenic Escherichia coli CFT073 , ATCC , Cat#700928.

Techniques: Infection, Comparison, MANN-WHITNEY

Journal: iScience

Article Title: Obesity promotes urinary tract infection by disrupting bladder focal adhesion kinase signaling

doi: 10.1016/j.isci.2025.113862

Figure Lengend Snippet: FAK is both required and sufficient for the UPEC invasion of human urothelial cells (A) Human bladder urothelial cells were transduced with retroviral particles containing empty vector (Empty) or Flag-tagged FAK (FAK-OX). Top: Representative Western blot confirmed increased FLAG tag and FAK expression in FAK-OX cells. Bottom: Transduced cells were challenged with UPEC. Shown are the percentages of bacteria invading the cells. (B) Human urothelial cells were transfected with non-targeting siRNA control (siNTC) or PTK2-targeting siRNA (siPTK2), which encodes FAK. Top: Representative Western blot confirms FAK knockdown. Bottom: Transfected cells were challenged with UPEC, and the percentage of invading UPEC is shown. (C) Human urothelial cells were transfected with HA-tagged wild-type AKT (WT) or membrane-targeted AKT (M/P). Top: Representative Western blot confirms HA tag expression and increased P-AKT expression. Bottom: Transfected cells were challenged with UPEC and shown, and the percentage of invading UPEC is shown. (A-C) Graphs show the mean and SEM. Results are from 3 to 9 independent experiments performed in triplicate ( n = 3–9). Asterisks indicate significant p- values (Student’s t test). ∗ p < 0.05.

Article Snippet: Uropathogenic Escherichia coli CFT073 , ATCC , Cat#700928.

Techniques: Transduction, Retroviral, Plasmid Preparation, Western Blot, FLAG-tag, Expressing, Bacteria, Transfection, Control, Knockdown, Membrane

Journal: Scientific Reports

Article Title: Biophysical and transcriptomic characterization of LL-37-derived antimicrobial peptide targeting multidrug-resistant Escherichia coli and ESKAPE pathogens

doi: 10.1038/s41598-025-22890-7

Figure Lengend Snippet: Antimicrobial efficacy of the peptide against various Escherichia coli strains. (A) Minimum inhibitory concentrations (MICs) determined for laboratory E. coli K-12, uropathogenic E. coli (UPEC), and multidrug-resistant (MDR) strain, following CLSI guidelines. Data are shown as mean ± SD from three independent biological replicates, with error bars indicating SD. (B) Time-kill assays showing bactericidal kinetics at four times the MIC concentration, with 90% bacterial reduction achieved within 2 h for all strains. Values represent mean CFU reduction rate ± SD from three independent replicates; error bars indicate SD. (C) Assessment of resistance development after 10 serial passages of each strain in sub-MIC peptide concentrations, indicating no significant increase in MIC and low potential for resistance emergence. MIC values are normalized to passage 1 baseline and presented as mean ± SD from three independent replicates, with error bars indicating SD.

Article Snippet: Bacterial strains used in this study included E. coli K-12 (ATCC® 10798TM, ATCC), uropathogenic E. coli CFT073 (ATCC® 700928TM, ATCC), multidrug-resistant (MDR) E. coli NCTC 13353TM (NCTC), Pseudomonas aeruginosa (ATCC® 27853TM, ATCC), Acinetobacter baumannii (ATCC® 19606TM, ATCC), Klebsiella quasipneumoniae (ATCC® 700603TM, ATCC), Staphylococcus aureus (ATCC® 25923TM, ATCC), and Enterococcus faecium (ATCC® 19434TM, ATCC).

Techniques: Concentration Assay

Journal: Scientific Reports

Article Title: Biophysical and transcriptomic characterization of LL-37-derived antimicrobial peptide targeting multidrug-resistant Escherichia coli and ESKAPE pathogens

doi: 10.1038/s41598-025-22890-7

Figure Lengend Snippet: Mechanism of membrane disruption and transcriptomic response to peptide treatment in E. coli strains. (A) Calcein dye leakage assay demonstrating selective membrane permeabilization of bacterial-mimetic POPE/POPG vesicles by the peptide, with minimal leakage observed in mammalian-mimetic POPC/cholesterol vesicles. Values expressed as % dye leakage relative to 0.1% Triton X-100 (100% release) and shown as mean ± SD from three independent experiments, with error bars indicating SD. (B) RNA sequencing analysis showing upregulation of stress response and efflux pump genes and downregulation of growth-related genes in E. coli K-12, UPEC, and MDR strains after sub-MIC peptide exposure. Data presented as log₂ fold change relative to untreated control; mean ± SE of three biological replicates, with error bars indicating SE. (C) KEGG pathway enrichment analysis highlighting activation of stress response and protein folding pathways alongside repression of DNA replication and cell division pathways across the three E. coli strains. Data represent mean enrichment scores ± SD from three replicates, with error bars indicating SD; enrichment significance adjusted by Benjamini–Hochberg correction (FDR < 0.05).

Article Snippet: Bacterial strains used in this study included E. coli K-12 (ATCC® 10798TM, ATCC), uropathogenic E. coli CFT073 (ATCC® 700928TM, ATCC), multidrug-resistant (MDR) E. coli NCTC 13353TM (NCTC), Pseudomonas aeruginosa (ATCC® 27853TM, ATCC), Acinetobacter baumannii (ATCC® 19606TM, ATCC), Klebsiella quasipneumoniae (ATCC® 700603TM, ATCC), Staphylococcus aureus (ATCC® 25923TM, ATCC), and Enterococcus faecium (ATCC® 19434TM, ATCC).

Techniques: Membrane, Disruption, RNA Sequencing, Control, Activation Assay

Journal: PLOS Pathogens

Article Title: Single-cell analysis reveals an important role of CD137L + macrophages in the host response to uropathogenic Escherichia coli infection in the bladder

doi: 10.1371/journal.ppat.1013543

Figure Lengend Snippet: (A) Graphical illustration of the experimental setup. Bladders were harvested from both UPEC and control groups (in duplicate), then enzymatically digested to generate single-cell suspensions. Live CD45 + cells were FACS-sorted and loaded cells for scRNA-seq. This figure was created using Figdraw and is licensed with authorization from Figdraw ( www.figdraw.com ). (B) Nonlinear dimensionality reduction Uniform Manifold Approximation (UMAP) visualization of 24415 bladder CD45 + immune cells identified 13 different cell types after unsupervised clustering in healthy and UPEC infection group, respectively. Each point depicts a single cell, colored according to cell type designation. (C) Dot plots of gene expression level identified within CD45 + immune cell populations. (D) Summary of proportion of assigned cell types in healthy and UPEC infection group, respectively. (E) UMAP visualization of 16 distinct bladder monocyte clusters, myeloid populations colored in accordance of group. (F) Dot plots of gene expression level identified within myeloid populations. (G) Bar graph of relative abundance of each cluster of myeloid types healthy and UPEC infection conditions. (H) Differences in selected hallmark pathway activities scored with GSVA software. (I) Dot plots displaying the representative differentially enriched GOBP terms between Ms_2 ( Ccl2 hi Ms) and Ms_5 ( Tnfsf9 + Ms). (J) GSEA reveals bacterial infection associated signal pathways enriched in Ms_5 ( Tnfsf9 + Ms) compared with Ms_2 ( Ccl2 hi Ms).

Article Snippet: Uropathogenic Escherichia coli (UPEC) strain CFT073 was purchased from American type culture collection (700928).

Techniques: Control, Infection, Gene Expression, Software

Journal: PLOS Pathogens

Article Title: Single-cell analysis reveals an important role of CD137L + macrophages in the host response to uropathogenic Escherichia coli infection in the bladder

doi: 10.1371/journal.ppat.1013543

Figure Lengend Snippet: (A) CD137L + Ms in bladders from 8-week-old mice in UPEC and Control groups were analyzed by flow cytometry. Dot plots depict the gating strategy for CD137L + Ms. Graph shows the proportion of bladder CD137L + Ms ( n = 5). (B) CD137L + Ms in bladders from 8-week-old infected Tnfsf9 F/F Lyz2 +/+ and Tnfsf9 F/F Lyz2 Cre/+ mice were analyzed by flow cytometry. Dot plots depict the gating strategy for CD137L + Ms. Graph shows the proportion of bladder CD137L + Ms (n = 5). (C) Bacterial load was assessed 24 hours after infection ( n = 10). (D) The mRNA expression of Il1b , Il6 and Tnf in the bladder tissues were measured by RT-PCR ( n = 6). (E) H&E Staining of bladders from 8-week-old female Tnfsf9 F/F Lyz2 +/+ and Tnfsf9 F/F Lyz2 Cre/+ mice. Histology scores were assessed after infection ( n = 5). (F) Representative images of uroplakin3a in superficial bladder urothelium of Tnfsf9 F/F Lyz2 +/+ and Tnfsf9 F/F Lyz2 Cre/+ UTIs mice models ( n = 4). (G) The expression of IL-1β and TNF-α on macrophages between infected Tnfsf9 F/F Lyz2 +/+ and Tnfsf9 F/F Lyz2 Cre/+ mice were analyzed by flow cytometry. The graph depicts the expression of IL-1β and TNF-α on macrophages (n = 6).

Article Snippet: Uropathogenic Escherichia coli (UPEC) strain CFT073 was purchased from American type culture collection (700928).

Techniques: Control, Flow Cytometry, Infection, Expressing, Reverse Transcription Polymerase Chain Reaction, Staining

Journal: PLOS Pathogens

Article Title: Single-cell analysis reveals an important role of CD137L + macrophages in the host response to uropathogenic Escherichia coli infection in the bladder

doi: 10.1371/journal.ppat.1013543

Figure Lengend Snippet: (A) UMAP visualization of 16 distinct bladder lymphoid cells in UPEC and Control groups, colored according to cluster designation. (B) Dot plots of gene expression level identified within lymphoid cells. (C) Summary of proportion of assigned lymphoid cells in UPEC and Control groups, respectively. (D) Representative genes related to typical functions of Tregs in UPEC and Control groups. (E) Dot plots displaying the representative differentially enriched GOBP terms between Tregs taken before and after the infection. (F) GSEA reveals infection-related pathways enriched in Tregs after UPEC infection. Each dot represents one mouse; lines are medians. (G) Tregs in bladders from 8-week-old mice in UPEC and Control groups were analyzed by flow cytometry. Graph shows the proportion of bladder Tregs ( n = 5). (H) Tregs in bladder of Foxp3 DTR with DT injection or not were analyzed by flow cytometry. Graph shows the proportion of bladder Tregs. Dot plots depict the gating strategy for Tregs ( n = 4). (I) Bacterial load was assessed 24 hours after infection ( n = 10). (J) The mRNA expression of Il1b , Il6 and Tnf in the bladder tissues were measured by RT-PCR ( n = 4). (K) H&E Staining of bladders from Foxp3- DTR and normal mice after UPEC infection. Histology scores were assessed after infection ( n = 5). (L) Representative images of uroplakin3a in superficial bladder urothelium of Foxp3 DTR and normal UTIs mice models ( n = 4).

Article Snippet: Uropathogenic Escherichia coli (UPEC) strain CFT073 was purchased from American type culture collection (700928).

Techniques: Control, Gene Expression, Infection, Flow Cytometry, Injection, Expressing, Reverse Transcription Polymerase Chain Reaction, Staining

Journal: PLOS Pathogens

Article Title: Single-cell analysis reveals an important role of CD137L + macrophages in the host response to uropathogenic Escherichia coli infection in the bladder

doi: 10.1371/journal.ppat.1013543

Figure Lengend Snippet: (A) Selected significant signaling pathways were ranked based on their differences in overall information flow within the inferred networks between UPEC and Control groups. The top signaling pathways colored red are more enriched in UPEC group, the middle one colored black is equally enriched in UPEC and Control groups, and the bottom ones colored green are more enriched in Control group. (B) Violin plot showing the expression distribution of signaling genes involved in the inferred CD137 signaling network in UPEC and Control groups. (C) Heatmap shows the relative importance of each cell group based on the computed four network centrality measures of CD137 signaling network. (D-E) The expression of CTLA-4 and PD-1 on Tregs between infected Tnfsf9 F/F Lyz2 +/+ and Tnfsf9 F/F Lyz2 Cre/+ mice were analyzed by flow cytometry. The graph depicts the expression of CTLA-4 and PD-1 on Tregs (n = 5). (F-G) The expression of CTLA-4 and PD-1 on Tregs between infected Tnfsf9 F/F Itagx +/+ and Tnfsf9 F/F Itagx Cre/+ mice were analyzed by flow cytometry. The graph depicts the expression of CTLA-4 and PD-1 on Tregs (n = 5).

Article Snippet: Uropathogenic Escherichia coli (UPEC) strain CFT073 was purchased from American type culture collection (700928).

Techniques: Protein-Protein interactions, Control, Expressing, Infection, Flow Cytometry